Project

Part:BBa_I3420:Design

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-28)


Test: Switch functionality and bistability (I3410.E0422.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 551
    Illegal BglII site found at 4788
    Illegal BglII site found at 5641
    Illegal BglII site found at 7540
    Illegal BamHI site found at 9241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 715
    Illegal SapI.rc site found at 5805
    Illegal SapI.rc site found at 7704


Design Notes

The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000).

Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432).

The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).



Source

References